Mass Spectrometry-based Proteomics with Post-Translational Modification Biology (Phospho & Glycoproteomics) (Online Advanced Training)

11 April-10 May 2026 | 6:00 PM IST | Online Training | Certificate

About the Training

This 5-week advanced online training focuses on mass spectrometry–based proteomics with Post-Translational Modification (PTM) biology, specifically phosphorylation and glycosylation. The program combines strong conceptual foundations with hands-on data analysis using real biological datasets. Participants will gain expertise in proteomics workflows, quantitative analysis, and PTM interpretation, with a special focus on understanding disease signaling pathways. The course is designed to prepare learners for research, biotech, and pharma R&D roles through practical exposure and case-based learning.

What You Will Learn

• Fundamentals of proteomics (top-down & bottom-up approaches)

• Working principles of mass spectrometry (Orbitrap)

• Sample preparation techniques in proteomics

• Quantitative proteomics and data normalization

• Hands-on MaxQuant & Perseus workflow

• Phosphoproteomics & glycoproteomics analysis

• PTM-based disease signaling and case studies

• Pathway analysis, Gene Ontology & network mapping (Cytoscape)

  
  

Course Features

• E-Certificate upon completion

• Live & interactive sessions

• Session recordings access

• E-materials & lecture PPTs

• Hands-on training with real datasets

• Tools covered: MaxQuant, Perseus, Cytoscape, pGlyco, GlycoWorkbench

  
  

Learning Outcomes

• Understand mass spectrometry–based proteomics workflows

• Perform quantitative analysis of proteomic datasets

• Identify and interpret phosphorylation & glycosylation PTMs

• Translate data into biological pathways and networks

• Apply skills in proteomics research, biotech, and pharma R&D

  
  

Who Can Join

• B.Sc / M.Sc / B.Tech / M.Tech students in Biotechnology, Life Sciences, Biochemistry, Microbiology

• PhD scholars working in proteomics, PTMs, or signaling biology

• Early-career researchers in omics data analysis

• Professionals aiming for careers in bioinformatics, proteomics & translational research

Course Fee

Indian Participants: ₹1899 INR
International Participants: $110 USD

Course Module

Week 1: Foundations of Proteomics

• Session 1:
  Introduction to Proteomics – Top-down & Bottom-up approaches
  Fundamentals of Mass Spectrometry – Orbitrap

• Session 2:
  Sample Preparation in Proteomics – Reduction, Alkylation, Digestion, Peptide Cleanup, and Peptide Labeling (importance & workflow)

Week 2: Proteomics Data Analysis

• Session 3:
  Data Analysis in Proteomics – MaxQuant workflow & introduction to Proteome Discoverer

• Session 4:
  Quantitative Proteomics – Normalization, Data Interpretation & Visualization using Perseus

Week 3: Phosphoproteomics

• Session 5:
  Introduction to Phosphoproteomics – Biology, site localization, and disease signaling

• Session 6:
  Phosphoproteomic Data Analysis – KSEA analysis, kinase-substrate mapping & kinome visualization (with cancer case studies)

Week 4: Glycoproteomics

• Session 7:
  Introduction to Glycoproteomics – Role in disease biology, especially cancer

• Session 8:
  Glycoproteomic Data Analysis – Oxonium ions, MS behavior, glycan & glycopeptide identification

Week 5: Biological Interpretation & Project

• Session 9:
  Biological Interpretation – Functional annotation, Gene Ontology, pathway enrichment & Cytoscape network mapping

• Session 10:
  Doubt Clarification + Capstone Project Initiation
  (Public dataset-based analysis: phosphoproteomics & glycoproteomics)

Software & Tools Covered

• MaxQuant

• Perseus

• Cytoscape

• pGlyco

• GlycoWorkbench

• Microsoft Excel